cat b 1325 (Vector Laboratories)

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Vector Laboratories cat b 1325
Cat B 1325, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 409 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cat+b+1325/Biotinylated+Lotus+Tetragonolobus+Lectin+(LTL)/pm41543936-750-285-283
Average 96 stars, based on 409 article reviews

cat b 1325 - by Bioz Stars, 2026-10

96/100 stars

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Article Title: NNMT inhibition counteracts tubular senescence and fibrosis in early stages of chronic kidney disease.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-human p21 (12D1) Cell Signaling Cat#2947; RRID: AB_823586 Rabbit anti-human NNMT Proteintech Cat#15123-1-AP; RRID: AB_2153579 Rat anti-mouse F4/80 Bio-Rad Cat#MCA497GA; RRID: AB_323806 Rat anti-mouse p21 [HUGO291] Abcam Cat#107099; RRID: AB_10891759 Rabbit anti-mouse p21 [EPR18021] Abcam Cat#188224; RRID: AB_2734729 Mouse anti-NNMT [3D8] Abcam Cat#119758; RRID: AB_10902083 Rabbit anti-αSMA Cell Signaling Cat#19245; RRID: AB_2734735 Rabbit anti-TWIST1 (E5G9Y) Cell Signaling Cat#90445; RRID: AB_3064916 Rabbit anti-Snail (C15D3) Cell Signaling Cat#3879S; RRID: AB_2255011 Rabbit anti-Vinculin (E1E9V) Cell Signaling Cat#13901; RRID: AB_2728768 Mouse anti-β actin Sigma-Aldrich Cat#A1978, RRID: AB_476692 Donkey anti-rabbit HRP Jackson ImmunoResearch Cat#711-035-152; RRID: AB_10015282 Donkey anti-mouse HRP Jackson ImmunoResearch Cat#715-035-150; RRID: AB_2340770 Donkey anti-rat HRP Jackson ImmunoResearch Cat#712-035-150; RRID: AB_2340638 Rabbit anti-rat SouthernBiotech Cat#6180-01; RRID: AB_2796254 Goat anti-rabbit/polyHRP Immunologic Cat#DPVR110HRP; RRID: AB_2915958 Mouse anti-panCK (AE-1/AE-3) AF647 Novus Biologicals Cat#NBP2-33200; RRID: AB_3284598 Mouse anti-megalin (H-10) AF488 Santa Cruz Biotech Cat#sc-515772; RRID: AB_2783023 Mouse anti-NNMT (OTI3D8) AF594 Novus Biologicals Cat#NBP2-00537; RRID: AB_3245049 Donkey anti-mouse IgG H + L AF488 Invitrogen Cat#A-21202; RRID: AB_141607 Donkey anti-Rabbit IgG H + L AF647 Invitrogen Cat#A-31573; RRID: AB_2536183 Biological samples Human DKD FFPE kidney biopsy sections Amsterdam University Medical Center (UMC) Biobank Assessment Committee BTC 2015.081 Human diabetic serum and urine samples ROCKIES clinical trial NCT04027530 Human diabetic serum and urine samples RED clinical trial NCT02682563 Human diabetic serum and urine samples RACELINES clinical trial NCT03433248 Chemicals, peptides, and recombinant proteins Nicotinamide (NAM) Sigma-Aldrich Cat#72340 5-amino-1-methylquinolinium iodide (NNMTi) Sigma-Aldrich Cat#SML2832 S-(5′-Adenosyl)-L-methionine iodide (SAM) Sigma-Aldrich Cat#A4377 rmIFNγ Prospec Cat#CYT-358 rhTGFβ Prospec Cat#CYT-716 CHIR-99021 R&D systems Cat#4423 rhFGF9 Peprotech Cat#100-23 Heparin Sigma-Aldrich Cat#H4784 rhEGF Sigma-Aldrich Cat#E9644 rhBMP7 Peprotech Cat#120-03P rhSDF1β R&D systems Cat#351-FS BSA Fraction V Roche Cat#10735094001 AGE-BSA Sigma Cat#121800-M SYTO 13 Thermo Fisher Cat#S7575 LTL-biotin Vector laboratories CatB-1325 (Continued on next page) Cell Reports 45, 116823, January 27, 2026 19 .. REAGENT or RESOURCE SOURCE IDENTIFIER Streptavidin AF405 Invitrogen Cat#S32351 rM-MLV Reverse Transcriptase Thermo Fisher Cat#28025013 Critical commercial assays MTT cell viability assay Roche Cat#11465007001 Albuwell M ELIS kit Exocell Cat#1011 QuantiChromTM Creatinine Assay Kit BioAssay Systems, Cat#DICT-500 Agencourt RNAdvance Tissue kit Beckman Coulter Life sciences Cat#A32646 Quant-iT RiboGreen RNA Assay Kit Thermo Fisher Cat#R11490 QuantSeq 3′ mRNA-Seq V2 Library Prep Kit FWD with UDI Lexogen Cat#193.384 Quant-iT PicoGreen dsDNA Assay Kit Thermo Fisher Cat#P7589 HS NGS Fragment Analysis kit Agilent Cat#DNF-474 P3 50 kit Illumina Cat#20046810 DNAdvance Tissue Kit Beckman Coulter Cat#A48705 Quant-iTTM PicoGreenTM dsDNA Assay Kit Thermo Fisher Cat#10398702 EZ-96 DNA Methylation MagPrep Kit Zymo Cat#D5040 Infinium MouseMethylation-12 v1.0 Illumina Cat#20042405 Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit Thermo Fisher Cat#C10635 CellEvent Senescence Green Flow Cytometry Assay Kit Thermo Fisher Cat#C10840 PureLink RNA Mini Kit Thermo Fisher Cat#12183018A NAD/NADH Quantification Kit Sigma-Aldrich Cat#MAK037 BCA assay kit Thermo Fisher Cat#23225 Deposited data Transcriptomics: NNMT inhibition in TECs This paper GSE280542 Transcriptomics: NAM and SAM supplementation in TECs This paper GSE280551 Transcriptomics: NNMT overexpression in TECs This paper GSE280552 Transcriptomics: young, old and NAM-supplemented old mice kidney This paper GSE280553 Transcriptomics: db/m and db/db mice kidney This paper GSE283610 DNA methylation array: NNMT inhibition and SAM supplementation in TECs This paper GSE310712 Experimental models: Cell lines Mouse TECs: conditionally immortalized proximal tubular epithelial cells Laboratory of Sandrine Florquin85 Cat#03069 Human iPSCs: induced pluripotent stem cells Provided by Jitske Jansen56 N/A Experimental models: Organisms/strains Mouse db/m and db/db: male BKS.Cg-Dock7m +/+ Leprdb/J, HE/HE and WT/HO, 7-week-old Charles River (JAX Laboratory) Cat#000642; RRID: IMSR_JAX:000642 Mouse wild-type (WT): male C57BL/6JRj, 4-months-old and 21-month-old Janvier Laboratories N/A Mouse wild-type (WT): male C57BL/6, 7-week-old Japan SLC, Inc. N/A Oligonucleotides See Table S1 for the RT-qPCR primer sequences This paper N/A Recombinant DNA pLenti-C-Myc-DDK-P2A-puro Origene Cat#PS100092V mouseNNMT-MYC-DDK-P2A-puro Origene Cat#MR203499L3V (Continued on next page) 20 Cell Reports 45, 116823, January 27, 2026

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